Nano Ultra-Performance Liquid Chromatography (UPLC)

Type of infrastructure:

Nano Ultra-Performance Liquid Chromatography (UPLC).

Technical description/model and performance:

Waters ACQUITY UPLC M-Class System for LC-MS analysis in nano scale. The system can generate a pulse-less, non-split flow in the range from 100 nl/min to 0.015 ml/min. It consists of a solvent manager, a sample manager (autosampler) for up to 96 samples and a trap valve manager.

Nano Ultra-Performance Liquid Chromatography.

Photo: Thomas Kieselbach

Field of application:

The Acquity-M nano-UPLC is currently installed at a HCTultra ETDII iontrap mass spectrometer from Bruker (funded by Vetenskapsrådet 822-2007-3473). This system is used for qualitative and quantitative peptide analysis in proteomics experiments at the KBC Proteomics facility.

Basic Mode of Operation:

A pressurized liquid solvent (e.g. 0.1% formic acid) containing the sample mixture is passed through a column filled with a solid hydrophobic adsorbent material. The components in the sample bind to the adsorbent material and are eluted by gradually increasing the concentration of an organic solvent (e.g. acetonitrile) in the liquid phase. The components dissociate from the solid phase according to their hydrophobicity, which leads to their separation .
Further information 

Location:

The instrument is placed at the Department of Chemistry, KBC Building house B, lab A4-41-01.

Access to the Instrumentation:

The instrument can be operated by trained staff. For more information, please contact Dr. Thomas Kieselbach, email: thomas.kieselbach@umu.se.

Other Information:

The instrumentation is a part of a nano LC-MS system and can only be used in that combination.

Name:

Thomas Kieselbach.

E-mail:

thomas.kieselbach@umu.se.

Ulf Sandqvist
2/19/2019