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Type of equipment:Optical Tweezers - instruments for micromanipulation and sub-pN resolution force measurement.
Technical description/model and performance:The instrument is built around an Olympus IX71 microscope.
Photo: Magnus Andersson
Field of application:The potential to measure: receptor-ligand interactions, biomechanical properties of macromolecules and polymers, viscosity in small volumes.
Basic Mode of Operation:A sample consisting of cells or microspheres is made simply and quickly on a microscope slide. In principle, a small flow cell with a volume of 5-100 µl is created. Using only laser light, the object can then be trapped and moved in 3D. By trapping micron-sized latex/polystyrene/glass beads, it is also possible to measure interaction forces. The beads then form a specific or non-specific attachment to e.g., a polymer. By separating the object, a measurable force is applied. The instrument can measure forces down to sub-pN resolution, i.e., <10^(-12) N.
Location:Floor 1, Physics building.
Access to the instrument:The instrument requires the help of technicians to be operated. Contact Magnus Andersson, Department of Physics.
Other information:We have developed our own software for use with the instrument.