High-Resolution Research Spectrophotometer and Multi-Mode Microplate Reader

Type of equipment:
High-Resolution Research Spectrophotometer and Multi-Mode Microplate Reader.



Technical description/model and performance:
Instrument 1: Horiba Aqualog standard 5500450521. Capacity: approx. 10-20 per hour depending on the concentration of dissolved organic carbon, see

Instrument 2: Multi-Mode Microplate Reader, Spectra Max M3 (Molecular devices). The spectrophotometer has the capacity to read various microtiter plates and even a cuvette holder for 1 cm pathlength cuvettes. Time consumption depends on application. For further information see:

Field of application:
Instrument 1: The instrument is used primarily to characterise dissolved organic material in natural water from watercourses, lakes and groundwater.
Instrument 2: The instrument is used for enzyme activity measurements in soil and water, as well as to characterise dissolved organic material in small volumes of groundwater.

Basic Mode of Operation:
Instrument 1: Water samples can either be placed directly in a cuvette and manually placed in the instrument's cuvette holder or in an autosampler connected to the instrument. In the latter case, a flow cuvette is used. The instrument can simultaneously measure excitation emission matrices (EEMs) and UV-Vis spectra. EEMs provide a two-dimensional visualisation of emission and excitation spectra, which can be used to characterise the dissolved organic material. There are also a number of other applications; see

Instrument 2: The instrument measures adsorbance and fluorescence in small volumes in microtiter plates with different numbers of wells (6 to 384 well plates). This allows the measurement of a large number of samples. For further information, see links on CIRC's website

Both instruments are located at the Climate Impact Research Centre in Abisko and constitute part of CIRC's research platform.

Access to the instrument:
Both instruments can be used by researchers at KBC who have undergone training on-site in Abisko or who have documented experience of using the instruments. The instruments can also be used, subject to availability, by national and international guest researchers in collaboration projects with CIRC.

Instrument 1: If you are interested in using the instrument, please contact Professor Reiner Giesler
Instrument 2: If you are interested in using the instrument, please contact Dr. Ellen Dorrepaal

Ulf Sandqvist